Environmental Biotechnology and Bioenergy

[CEST2019_00916] Co-Pyrolysis of Corn Stover Wıth Plastıc: Optımızatıon Based on Synergy
by Salvilla J., De Luna M., Rollon A.

The co-pyrolysis of lignocellulosic wastes such as Corn stover with plastic has been found to improve the both the yield and quality of pyrolysis oil and gas products. In this study, the co-pyrolysis of Corn stover (CS) with plastic (LDPE, and PP) were studied through thermogravimetric analysis. The parameter “change in weight loss rate” denoted by ΔW was used to quantify the synergistic effects on the yield during co-pyrolysis.

Session: 32, Room: B, at Fri, 09/06/2019 - 16:27 to 16:30
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00920] Hierarchization of pure silica LTA zeolite
by Tarach K. A., Valencia S., Góra-Marek K., Rey F.

The effect of base leaching on pure silica ITQ-29 zeolite being the counterpart of LTA zeolite, was investigated. The extent of the desilication process over pure silica zeolite was controlled by partial detemplation followed by silicon extraction. The potential of hierarchically porous ITQ-29 zeolite in environmentally friendly applications concern selective adsorption of organic molecules in aqueous environments. The introduction of additional porosity by desilication process offers full usage of zeolite grains volume without diffusional limitations during the adsorption process.

Session: 32, Room: B, at Fri, 09/06/2019 - 16:21 to 16:24
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00935] Bio-scrubber coupled with ozonation for enhanced VOCs abatement
by Senatore V., Oliva G., Zarra T., Belgiorno V., Naddeo V.

Volatile Organic Compounds (VOCs) are toxic for the environment and human health and their tendency to readily volatilize in the atmosphere leads to problems connected to odours annoyance. The conventional treatments for VOCs gaseous emissions conventionally entail the application of chemical-physical processes, only promoting the transfer of the contaminants from gas to liquid and/or solid phases. Advanced Oxidation Process (AOPs) and biological processes, conversely, support the oxidation of the organic pollutants, promoting their conversion into harmless and odourless compounds.

Session: 32, Room: B, at Fri, 09/06/2019 - 15:45 to 16:00
Oral presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00843] Heterotrophic growth of Chlorella vulgaris on crude glycerol
by Gougoulias N., Metsoviti M.N., Grigoriou M., Lamprakopoulos S., Mpesios A. , Papapolymerou G.

The heterotrophic growth of C. vulgaris using crude glycerol as the sole carbon source was studied in five 5L flasks. Air in each flask was provided at a rate of 300 L/hr and the contents were continuously stirred with a magnetic bar. Temperature, pH and initial inorganic nutrients (nitrogen, phosphorus, potassium and micronutrients) were kept the same in all flasks. The C/N ratio varied in each of the five flasks was set at 6, 13, 25.4, 61.5 and 118 respectively while the nitrogen initial concentration was equal to 45.4 mg/L in all flasks.

Session: 32, Room: B, at Fri, 09/06/2019 - 16:15 to 16:18
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00774] Pyrolysis of Spirulina sp. Microalgae: Effect of Temperature on Chemical Compositions of Bio-Oil and Aqueous Phase
by Özçakir G., Karaduman A.

Pyrolysis of Spirulina sp. Microalgae was carried out in a semi-batch glass reactor system. Effect of temperature on the yields of pyrolytic products (gaseous, liquid and solid residue) and chemical composition of the liquid products were investigated. All experiments were performed in 25 mL/min nitrogen atmosphere with 15 g feedstock which was dry and powder form of Spirulina. Temperature was varied from 470 to 620 °C with 50 °C break by utilizing PID controller which was setted 10 °C/min heating rate.

Session: 32, Room: B, at Fri, 09/06/2019 - 16:00 to 16:15
Oral presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00709] Anaerobic digestion of long-chain fatty acids (oleic, palmitic, stearic) with whey protein as the emulsifier
by Eftaxias A., Michailidis C., Stamatelatou K., Diamantis V., Aivasidis A.

Long chain fatty acids are major lipid constituents. In this study, anaerobic digestion of oleic, palmitic or stearic acid (5 g/L each) emulsified with whey protein (20 g/L) was examined in continuous (batch-fed) stirred tank digesters with 2 L working volume. Anaerobic co-digestion of oleic acid displayed high biogas yield (0.47 L/gCOD), compared to palmitic (0.42 L/gCOD) and stearic acid (0.30 L/gCOD). Oleic acid, despite its high biodegradability resulted in major inhibition of the acetoclastic methanogens, as demonstrated by VFA accumulation and by the methanogenic activity assay.

Session: 32, Room: B, at Fri, 09/06/2019 - 15:15 to 15:30
Oral presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00715] High-rate anaerobic co-digestion of agro-industrial wastes combined with ammonia recovery and biogas purification
by Eftaxias A., Georgiou D., Diamantis V., Koumara A., Koskinari M.D., Aivasidis A.

Screened dairy manure, pressure-sterilized animal by-products, and cheese whey were co-digested in a Plug Flow Reactor (PFR) over a period of 150 days. The PFR process was stable even under a hydraulic retention time of 3 days, corresponding to an organic loading rate (OLR) of 22 g/Ld. Effluent COD remained low (5.6±1.4 g/L) while VFA concentrations were negligible (< 0.5 g/L as COD). The biogas production rate from the PFR ranged from 2.6 up to 7.3 L/Ld.

Session: 32, Room: B, at Fri, 09/06/2019 - 15:30 to 15:45
Oral presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00718] Thermal and kinetic analysis of biomass fuel (powders) by differential thermal gravimetric analysis (TGA/DTG/DTA)
by Kumar P., Subbarao P.M.V., Kala L.D., Vijay V.K.

The kinetics of the thermal decomposition of the agriculture residues were evaluated using a thermo-gravimetric analyser under non-isothermal conditions. The thermal behaviour and pyrolysis of two types of biomass i.e. pearl millet cob and eucalyptus by using TGA from ambient to 1000°C. Three different heating rates (10, 15, and 20°C/min) were taken for the thermogravimetric analysis.

Session: 26, Room: B, at Fri, 09/06/2019 - 13:39 to 13:42
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00634] An innovative green iron-fertilizer, produced biotechnologically, for correcting iron chlorosis of soybean plants grown in calcareous soils
by Ferreira C., Lopez-Rayo S., Lucena J., Soares E., Soares H.

Iron deficiency is one of the main causes of chlorosis in plants, which leads to the loss in the field crops quality and yield. Iron-deficiency is a worldwide problem, particularly sever in calcareous soils (about 30% of world´s land surface). The current use of synthetic iron-chelates to prevent or correct iron-deficiency in plants raises environmental concerns due to their poor biodegradability. Thus, new, more environmentally-friendly efficient solutions are needed to solve iron deficiency-induced chlorosis (IDIC) in crops grown in calcareous soils.

Session: 32, Room: B, at Fri, 09/06/2019 - 15:00 to 15:15
Oral presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00607] Lipid production by Rhodosporidium toruloides growing on media presenting composition similarities with the spent sulfite liquor in batch and fed-batch cultures
by Michou S., Vastaroucha E.-S., Sarris D., Kalantzi O., Papanikolaou S.

Aim of this study is to explore the effect of sodium lignosulfonate (SL), a paper industry by-product, on cell growth and lipid production by the yeast Rhodosporidium toruloides, cultivated on xylose-based media, that mimic the principal waste-stream originated from paper production facilities (viz. the spent-sulfite liquor). Yeast lipids present increasing interest as alternative non-food feedstocks for biodiesel production. Strains DSM 4444 and NRRL Y-27012 were shake-flask cultured under nitrogen-limiting conditions using xylose at 50 g/L, and SL was added at varying concentrations.

Session: 26, Room: B, at Fri, 09/06/2019 - 13:36 to 13:39
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00610] Biotechnological production of polyols through conversions of crude glycerol by newly isolated strains of the yeast Yarrowia lipolytica
by Vastaroucha E.-S., Michou S., Kalantzi O., Papanikolaou S.

The purpose of this study is to investigate the ability of newly isolated Yarrowia lipolytica strains to grow on crude glycerol, the main by-product of the industrial production of biodiesel. In particular, the ability of the yeasts to metabolize glycerol and produce dry cell weight (DCW) and secondary metabolites such as lipid, endopolysaccharides and polyols (e.g. mannitol, arabitol, erythritol) was assessed.

Session: 26, Room: B, at Fri, 09/06/2019 - 13:42 to 13:45
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00593] LED light tailoring in a planar photobioreactor for optimization of microalgae growth.
by Riggio V.A., Occhipinti A., Grinic D., Zanetti M.

Microalgae are well known for their potential in producing valuable substances for nutraceutical and pharmaceutical industries, as well as a source of biofuel. The use of the photosynthetic capacity of microalgae is a new alternative for carbon dioxide bio-fixation. Therefore, the purpose of this work is to identify the best microalgae growth conditions, using an experimental planar photobioreactor (PBR) and LEDs with variable intensities as light source. Two different illumination intensity levels were used during the experiment: 33 and 57 µE of PPFD (Photosynthetic Photon Flux Density).

Session: 26, Room: B, at Fri, 09/06/2019 - 13:15 to 13:30
Oral presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00541] Screening strains of genus Pleurotus for biomass production in solid state fermentation of agricultural residues
by Diamantopoulou P., Melanouri E.-M., Papanikolaou S.

The ability of several strains belonging to Pleurotus ostreatus and P. eryngii mushroom genera to grow on various agricultural residues was examined and their mycelium growth rates and biomass production (estimated as glucosamine content) were compared. Four P. ostreatus (AMRL 137, 144, 147, 150) and four P. eryngii (AMRL 160, 163, 166, 173-6) strains were cultivated on wheat, barley and oat straw, poplar and beech-wood sawdust, cotton and coffee residues, corn-cobs, rice bark, olive cake supplemented with wheat bran on a final C/N 20-30.

Session: 32, Room: B, at Fri, 09/06/2019 - 16:24 to 16:27
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00512] Biotechnological conversions of crude glycerol, residue deriving from biodiesel production facilities, by strains of the yeast Yarrowia lipolytica
by Papanikolaou S., Diamantopoulou P., Sarris D., Aggelis G.

Aim of the present study was to assess the ability of four Yarrowia lipolytica strains (ACA-DC 50109, LFMB Y-20, ATCC 20460 and LMBF Y-45) to grow on biodiesel-derived crude glycerol, the principal residue-stream deriving from biodiesel manufacture. Initial trials were carried out in shake-flasks under nitrogen limitation (initial glycerol Glol0~40 g/L, initial nitrogen ~0.35 g/L), that favor the production of cellular lipids and/or extra-cellular secondary metabolites like citric acid (CA). All strains produced appreciable dry cell weight (DCW) quantities (up to 13.0 g/L).

Session: 26, Room: B, at Fri, 09/06/2019 - 13:33 to 13:36
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00368] Fuel performance of biodiesel from microalgae
by Cercado A.P., Ballesteros F., Capareda S.

Fuel performance of biodiesel produced from transesterification of microalgae was evaluated to assess its potential as alternative fuel in diesel engine. The biodiesel was produced from transesterification of microalgae Chlorella Vulgaris using K-pumice as catalyst. The engine used in the study was Yanmar 3009D, a small diesel engine with an output power rating of 14.2 kilowatts. The experiments showed comparable power and torque when the engine was run using both the commercial diesel and the algal biodiesel.

Session: 26, Room: B, at Fri, 09/06/2019 - 13:00 to 13:15
Oral presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00373] Cloning and sequencing of the gene encoding the enzyme for the reductive cleavage of diaryl ether bonds of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in Geobacillus thermodenitrificans UZO 3
by Suzuki Y., Nakamura M., Otsuka Y., Sato-Izawa K., Hishiyama S., Kameyama K., Kameyama T., Takahashi A., Katayama Y.

We have previously reported that a cell-free extract prepared from Geobacillus thermodenitrificans UZO 3 reductively cleaves diaryl ether bonds of 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD), a dioxin with the highest toxicity, in a sequential fashion producing 3’,4’,4,5-tetrachloro-2-hydroxydiphenyl ether (TCDE) as the intermediate, and 3,4-dichlorophenol (DCP) as the final reaction product. The detection of TCDE implicated the discovery of an unprecedented dioxin-degrading enzyme that reductively cleaves the diaryl ether bonds.

Session: 26, Room: B, at Fri, 09/06/2019 - 13:30 to 13:33
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00346] Characterization of Xylanase-Treated Karagumoy Fiber Reinforced Composite (KFRC)
by Mina M.T.M., Tumolva T.P.

This work determined the effects of enzyme treatment on the mechanical properties of natural fiber-reinforced composite using Pandanus simplex (karagumoy) fibers. Physical modification using enzyme improved chemical and mechanical properties of fibers and the karagumoy fiber-reinforced composites (KFRCs) produced. Enzyme concentration and soaking time were used as treatment parameters. The treatment scheme improved the mechanical properties of fibers and composites, as well as, the composites’ water absorption property.

Session: 32, Room: B, at Fri, 09/06/2019 - 16:18 to 16:21
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00278] Second generation bioethanol production from household food wastes via a newly isolated yeast strain of Wickerhamomyces anomalus
by Dazea D., Antonopoulou G., Alexandropoulou M., Ben Atitallah I., Mechichi T., Ntaikou I., Lyberatos G.

In the current study the efficiency of second generation bioethanol production from the pre-dried and shredded organic fraction of household food waste was investigated using the newly isolated yeast Wickerhamomyces anomalus X19. Separatete hydrolysis and fermentation (SHF) as well as simultaneous saccharification and fermentation (SSF) experiments were conducted at batch mode.

Session: 26, Room: B, at Fri, 09/06/2019 - 12:45 to 13:00
Oral presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00222] Dairy wastewater as growth substrate for biomass and biocompound production by Spirulina platensis
by Escolà G., Zapata D., Aristizábal A., Arroyave C., Poschenrieder C., Llugany M.

Spirulina former Arthrospira is a Cyanobacteria with economic applications in agriculture, biofuel production and wastewater treatment. In this study the influence of the carbon source in the medium and the medium’s conductivity for Spirulina platensis UTEX growth over time was investigated. Three different culture media were evaluated: i) modified UTEX medium with inorganic carbon in the form of sodium bicarbonate and carbonate, ii) modified UTEX medium where inorganic carbon was replaced by lactic acid, and sea water enriched with lactic acid.

Session: 26, Room: B, at Fri, 09/06/2019 - 12:30 to 12:45
Oral presentation in Environmental Biotechnology and Bioenergy