Session 26 - Environmental Biotechnology and Bioenergy

[CEST2019_00718] Thermal and kinetic analysis of biomass fuel (powders) by differential thermal gravimetric analysis (TGA/DTG/DTA)
by Kumar P., Subbarao P.M.V., Kala L.D., Vijay V.K.

The kinetics of the thermal decomposition of the agriculture residues were evaluated using a thermo-gravimetric analyser under non-isothermal conditions. The thermal behaviour and pyrolysis of two types of biomass i.e. pearl millet cob and eucalyptus by using TGA from ambient to 1000°C. Three different heating rates (10, 15, and 20°C/min) were taken for the thermogravimetric analysis.

Session: 26, Room: B, at Fri, 09/06/2019 - 13:39 to 13:42
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00607] Lipid production by Rhodosporidium toruloides growing on media presenting composition similarities with the spent sulfite liquor in batch and fed-batch cultures
by Michou S., Vastaroucha E.-S., Sarris D., Kalantzi O., Papanikolaou S.

Aim of this study is to explore the effect of sodium lignosulfonate (SL), a paper industry by-product, on cell growth and lipid production by the yeast Rhodosporidium toruloides, cultivated on xylose-based media, that mimic the principal waste-stream originated from paper production facilities (viz. the spent-sulfite liquor). Yeast lipids present increasing interest as alternative non-food feedstocks for biodiesel production. Strains DSM 4444 and NRRL Y-27012 were shake-flask cultured under nitrogen-limiting conditions using xylose at 50 g/L, and SL was added at varying concentrations.

Session: 26, Room: B, at Fri, 09/06/2019 - 13:36 to 13:39
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00610] Biotechnological production of polyols through conversions of crude glycerol by newly isolated strains of the yeast Yarrowia lipolytica
by Vastaroucha E.-S., Michou S., Kalantzi O., Papanikolaou S.

The purpose of this study is to investigate the ability of newly isolated Yarrowia lipolytica strains to grow on crude glycerol, the main by-product of the industrial production of biodiesel. In particular, the ability of the yeasts to metabolize glycerol and produce dry cell weight (DCW) and secondary metabolites such as lipid, endopolysaccharides and polyols (e.g. mannitol, arabitol, erythritol) was assessed.

Session: 26, Room: B, at Fri, 09/06/2019 - 13:42 to 13:45
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00593] LED light tailoring in a planar photobioreactor for optimization of microalgae growth.
by Riggio V.A., Occhipinti A., Grinic D., Zanetti M.

Microalgae are well known for their potential in producing valuable substances for nutraceutical and pharmaceutical industries, as well as a source of biofuel. The use of the photosynthetic capacity of microalgae is a new alternative for carbon dioxide bio-fixation. Therefore, the purpose of this work is to identify the best microalgae growth conditions, using an experimental planar photobioreactor (PBR) and LEDs with variable intensities as light source. Two different illumination intensity levels were used during the experiment: 33 and 57 µE of PPFD (Photosynthetic Photon Flux Density).

Session: 26, Room: B, at Fri, 09/06/2019 - 13:15 to 13:30
Oral presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00512] Biotechnological conversions of crude glycerol, residue deriving from biodiesel production facilities, by strains of the yeast Yarrowia lipolytica
by Papanikolaou S., Diamantopoulou P., Sarris D., Aggelis G.

Aim of the present study was to assess the ability of four Yarrowia lipolytica strains (ACA-DC 50109, LFMB Y-20, ATCC 20460 and LMBF Y-45) to grow on biodiesel-derived crude glycerol, the principal residue-stream deriving from biodiesel manufacture. Initial trials were carried out in shake-flasks under nitrogen limitation (initial glycerol Glol0~40 g/L, initial nitrogen ~0.35 g/L), that favor the production of cellular lipids and/or extra-cellular secondary metabolites like citric acid (CA). All strains produced appreciable dry cell weight (DCW) quantities (up to 13.0 g/L).

Session: 26, Room: B, at Fri, 09/06/2019 - 13:33 to 13:36
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00368] Fuel performance of biodiesel from microalgae
by Cercado A.P., Ballesteros F., Capareda S.

Fuel performance of biodiesel produced from transesterification of microalgae was evaluated to assess its potential as alternative fuel in diesel engine. The biodiesel was produced from transesterification of microalgae Chlorella Vulgaris using K-pumice as catalyst. The engine used in the study was Yanmar 3009D, a small diesel engine with an output power rating of 14.2 kilowatts. The experiments showed comparable power and torque when the engine was run using both the commercial diesel and the algal biodiesel.

Session: 26, Room: B, at Fri, 09/06/2019 - 13:00 to 13:15
Oral presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00373] Cloning and sequencing of the gene encoding the enzyme for the reductive cleavage of diaryl ether bonds of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in Geobacillus thermodenitrificans UZO 3
by Suzuki Y., Nakamura M., Otsuka Y., Sato-Izawa K., Hishiyama S., Kameyama K., Kameyama T., Takahashi A., Katayama Y.

We have previously reported that a cell-free extract prepared from Geobacillus thermodenitrificans UZO 3 reductively cleaves diaryl ether bonds of 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD), a dioxin with the highest toxicity, in a sequential fashion producing 3’,4’,4,5-tetrachloro-2-hydroxydiphenyl ether (TCDE) as the intermediate, and 3,4-dichlorophenol (DCP) as the final reaction product. The detection of TCDE implicated the discovery of an unprecedented dioxin-degrading enzyme that reductively cleaves the diaryl ether bonds.

Session: 26, Room: B, at Fri, 09/06/2019 - 13:30 to 13:33
Flash presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00278] Second generation bioethanol production from household food wastes via a newly isolated yeast strain of Wickerhamomyces anomalus
by Dazea D., Antonopoulou G., Alexandropoulou M., Ben Atitallah I., Mechichi T., Ntaikou I., Lyberatos G.

In the current study the efficiency of second generation bioethanol production from the pre-dried and shredded organic fraction of household food waste was investigated using the newly isolated yeast Wickerhamomyces anomalus X19. Separatete hydrolysis and fermentation (SHF) as well as simultaneous saccharification and fermentation (SSF) experiments were conducted at batch mode.

Session: 26, Room: B, at Fri, 09/06/2019 - 12:45 to 13:00
Oral presentation in Environmental Biotechnology and Bioenergy
[CEST2019_00222] Dairy wastewater as growth substrate for biomass and biocompound production by Spirulina platensis
by Escolà G., Zapata D., Aristizábal A., Arroyave C., Poschenrieder C., Llugany M.

Spirulina former Arthrospira is a Cyanobacteria with economic applications in agriculture, biofuel production and wastewater treatment. In this study the influence of the carbon source in the medium and the medium’s conductivity for Spirulina platensis UTEX growth over time was investigated. Three different culture media were evaluated: i) modified UTEX medium with inorganic carbon in the form of sodium bicarbonate and carbonate, ii) modified UTEX medium where inorganic carbon was replaced by lactic acid, and sea water enriched with lactic acid.

Session: 26, Room: B, at Fri, 09/06/2019 - 12:30 to 12:45
Oral presentation in Environmental Biotechnology and Bioenergy